software, algorithm geneious Search Results


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Biomatters Ltd geneious prime
A phylogenetic tree depicting the evolutionary relationships among selected LSDV isolates. Complete DNA polymerase gene sequences were extracted from representative LSDV strains across various countries and aligned using MAFFT (v7.450) with the G–INS–I algorithm (BLOSUM62 scoring matrix; gap open penalty: 1.53; offset value: 0.123) in <t>Geneious</t> Prime (v23.1.1, Biomatters, Ltd., Auckland, New Zealand). A maximum likelihood (ML) tree was constructed in Geneious Prime with 1000 bootstrap replicates, using an avipoxvirus sequence (GenBank accession no. KX857215 ) as the outgroup. The branch tip labels indicate GenBank accession numbers followed by the country of origin. LSDVs sequenced in this study and previously reported Bangladeshi isolates are highlighted in pink and blue, respectively. The tree was visualised using FigTree v1.4.4, with an automatic scale bar added. Bootstrap values are displayed as percentages, with values below 80% omitted.
Geneious Prime, supplied by Biomatters Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A phylogenetic tree depicting the evolutionary relationships among selected LSDV isolates. Complete DNA polymerase gene sequences were extracted from representative LSDV strains across various countries and aligned using MAFFT (v7.450) with the G–INS–I algorithm (BLOSUM62 scoring matrix; gap open penalty: 1.53; offset value: 0.123) in Geneious Prime (v23.1.1, Biomatters, Ltd., Auckland, New Zealand). A maximum likelihood (ML) tree was constructed in Geneious Prime with 1000 bootstrap replicates, using an avipoxvirus sequence (GenBank accession no. KX857215 ) as the outgroup. The branch tip labels indicate GenBank accession numbers followed by the country of origin. LSDVs sequenced in this study and previously reported Bangladeshi isolates are highlighted in pink and blue, respectively. The tree was visualised using FigTree v1.4.4, with an automatic scale bar added. Bootstrap values are displayed as percentages, with values below 80% omitted.

Journal: Viruses

Article Title: Integrating Serological and Genomic Data to Elucidate Lumpy Skin Disease Virus Diversity in Cattle from Bangladesh

doi: 10.3390/v17081126

Figure Lengend Snippet: A phylogenetic tree depicting the evolutionary relationships among selected LSDV isolates. Complete DNA polymerase gene sequences were extracted from representative LSDV strains across various countries and aligned using MAFFT (v7.450) with the G–INS–I algorithm (BLOSUM62 scoring matrix; gap open penalty: 1.53; offset value: 0.123) in Geneious Prime (v23.1.1, Biomatters, Ltd., Auckland, New Zealand). A maximum likelihood (ML) tree was constructed in Geneious Prime with 1000 bootstrap replicates, using an avipoxvirus sequence (GenBank accession no. KX857215 ) as the outgroup. The branch tip labels indicate GenBank accession numbers followed by the country of origin. LSDVs sequenced in this study and previously reported Bangladeshi isolates are highlighted in pink and blue, respectively. The tree was visualised using FigTree v1.4.4, with an automatic scale bar added. Bootstrap values are displayed as percentages, with values below 80% omitted.

Article Snippet: Sequence similarity between the selected LSDV sequences and reference genomes was evaluated through multiple sequence alignment using the MAFFT L-INS-I algorithm, implemented within Geneious Prime (Biomatters, Ltd., Auckland, New Zealand).

Techniques: Construct, Sequencing